Coding

Part:BBa_K888007:Design

Designed by: Juan Quijano   Group: iGEM12_UANL_Mty-Mexico   (2012-09-25)

rhMT (recombinant human metallothionein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has the following prefix sites, EcoRI and BglII and as suffix sites, BamHI and SpeI. Through this way, it is possible to obtain fusion proteins in frame via BglII and BamHI sites, and also enables the use of Standard Assembly 10 vectors.


Source

human (recombinant) (Ngu, TT and Stillman, MJ, 2006)

References

Ngu, TT and MJ Stillman. (2006). Arsenic Binding to Human Metallothionein. J. Am. Chem. Soc. 128 (38), 12473-12483.